minimal essential medium emem (ATCC)

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ATCC minimal essential medium emem
Minimal Essential Medium Emem, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/emem+medium/Eagle's+Minimum+Essential+Medium/pmc13156593-197-18-22
Average 99 stars, based on 6330 article reviews

minimal essential medium emem - by Bioz Stars, 2026-09

99/100 stars

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Cell Culture:

Article Title: GSPT1-specific protein degradation is effective in preclinical models of chemoresistant MYCN -amplified neuroblastoma
Article Snippet: Human neuroblastoma Kelly cell line (DSMZ, #ACC 355) was cultured in RPMI 1640 medium (Gibco, #21875-034), 10% FBS (Gibco, #10500-064) and 1% Penicillin-Streptomycin (Biowest, #L0022-100). .. Hep3B cell line was cultured in EMEM medium (ATCC, #30-2003) supplemented with 10% FBS (Gibco, #10500-064) and 1% Penicillin-Streptomycin (Biowest, #L0022-100). .. Snap-frozen tumor pieces were incubated at -20 °C in RNAlater-ICE (Invitrogen, #AM7030) for a minimum of 24 h before extraction.

Article Title: EBV Triggers a Distinct Antiviral Response in HMC3 Cells
Article Snippet: .. Human microglial HMC3 cells (ATCC, Cat#CRL-3304) were cultured in EMEM medium (ATCC, Cat30-2003) supplemented with 10% FBS and 1% pen/strep (Gibco, Cat#15140-122), hereafter referred to as complete EMEM (cEMEM). ..

Article Title: Preeclampsia‐derived small extracellular vesicles disrupt blood–brain barrier integrity and activate glial cells <i>in vitro</i>
Article Snippet: .. The human microglia cell line HMC3, also obtained from the ATCC (Cat. No. CRL-3304), was cultured in EMEM medium (ATCC, Cta. ..

Article Title: Improve the Efficacy of B7-H3-Targeting Antibody-Drug Conjugate DS-7300a in TP53-deficient Tumors by Inducing Ferroptosis
Article Snippet: 22RV1 and LNCaP cells were cultured in RPMI-1640 medium (Corning, 10-040-CV) with 10% FBS (Thermo Scientific, 10082147) and 1% penicillin-streptomycin (Thermo Scientific, 15140163). .. DU145 was cultured in EMEM medium (ATCC, 30-2003) with 10% FBS (Thermo Scientific, 10082147) and 1% penicillin-streptomycin (Thermo Scientific, 15140163). .. Cell lines were authenticated by MD Anderson’s Cytogenetic and Cell Authentication Core and were regularly tested for mycoplasma using MycoAlert PLUS detection kit (Lonza, LT07-710), according to the manufacturer’s instructions.

Article Title: Human epidermal growth factor receptor 3-targeted near-infrared photoimmunotherapy in a xenograft mouse model of breast cancer.
Article Snippet: .. MDA-MB-468 Luc cells were cultured in RPMI 1640 medium (Cat# 11875119, Thermo Fisher Scientific), and MCF-7 cells were cultured in EMEM medium (Cat# 30-2003, ATCC, Manassas, VA, USA), each supplemented with 10% fetal bovine serum (Cat# A5670801, Thermo Fisher Scientific), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Cat# 15140122, Thermo Fisher Scientific), in a humidified incubator in an atmosphere of 95% air and 5% carbon dioxide at 37°C. .. Both MDA-MB-468 Luc and MCF-7 cell lines were authenticated by IDEXX BioAnalytics (Westbrook, ME, USA) via STR profiling in August 2023.

Article Title: ESRP2 constrains EMT plasticity associated with ZEB1 expression in bladder cancer
Article Snippet: Cell lines were cultured in an appropriate growth medium supplemented with 10% fetal bovine serum (Gibco, Darmstadt, Germany, 10500064) at 37°C and 5% CO 2 . .. RT-4 and T-24 cell lines were cultured in McCoy’s 5A medium (ATCC, 30-2007), the 5637 line in RPMI-1640 (ATCC, 30-2001), and UM-UC-3 in EMEM medium (ATCC, 30-2003). ..

Expressing:

Article Title: Combination of LILRB1/2 pathway inhibitors and PD-1 pathway inhibitors
Article Snippet: .. Parental SK-MEL-2 (SK2) were maintained EMEM medium (ATCC) with 10% FBS and 1% penicillin/streptomycin at 37° C. Cells with stable expression of HLA-G were established by retroviral transduction. .. The HLA-G. β2 microglobulin cDNA was inserted into retrovirus vector pLHCX and was co-transfected with Lipofectamine 3000 (Thermo Fisher Scientific) into GP2-293 packaging cells with pVSV-G. Supernatant were collected 48 hours after transfection and filtered.

Retroviral:

Transduction:

Multiple Displacement Amplification:

Article Title: Selective Pyroptosis in NF1-Deficient Cells through PKCδ Agonism
Article Snippet: .. Breast cancer and other cancer cell lines were maintained at 37°C in a 5% CO 2 atmosphere, with the following media specifications: SK-MES-1: EMEM medium (ATCC, #30-2003) supplemented with 10% FBS; MCF-7, MDA-MB-468, MDA-MB-231, and HCT-116: DMEM medium (Thermo Fisher, #11965092) supplemented with 10% FBS. ..

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